Application of the acoustic method in biomass estimation of multi-species fisheries in the South China Sea Rosidi bin Ali*1, Raja Bidin bin Raja Hassan*1, Albert Chuan Gambang*2, Samsudin bin Basir*3, Hadil bin Rajali*2, Kunimune Shiomi*1 and Shunji Fujiwara*1 *1 SEAFDEC/MFRDMD, Fisheries Garden, Chendering, 21080 Kuala Terengganu, Terengganu, MALAYSIA *2 Fisheries Research Institute, Bintawa, 93744 Kuching, Sarawak, MALAYSIA *3 Fisheries Research Institute, 11960 Batu Maung, Penang, MALAYSIA This paper presents the approaches and the findings of the acoustic surveys in estimating the fish biomass of multi-species fisheries, in waters of the Gulf of Thailand waters and the east coast of Peninsular Malaysia, based on two acoustic surveys that were carried out with M/V SEAFDEC in those areas. The first survey was conducted on the 5.9.1995 yp 28.5.1995(i.e. the pre-Northeast monsoon season), and the second survey was done on the 24.4.1996 to 17.5.1996 (i.e. the post-Northeast monsoon season). The scientific echo-sounder installed on board M/V SEAFDEC, FQ-70 (Furuno Electric Co.), was employed in the surveys. The erroneous SV values collected by the 200 kHz transducer were careful examined and were deleted. For the pelagic fish, there was a significant difference in the distribution of SV values of the pre-monsoon compared to the values of the post-monsoon. The high SVs were observed in the pre-monsoon survey, from the off-shore to the near shore waters. However, the high SVs was not detected in the post-monsoon survey. In case of the demersal fish, there was no significant difference in the distribution of SV values of the pre-monsoon compared to the values of the post-monsoon. The higher SVs were observed in the northern part of the Gulf of Thailand and a long the east coast of Peninsular Malaysia. For the biomass estimation, the representative species of demersal fish and pelagic fish from the Gulf of Thailand and the representative species of demersal fish and pelagic fish from the east coast of Peninsula Malaysia were determined separately. The information on length and weight of these species were gathered from the annual catch statistics and from the in-situ surveys at the landing place. The target strength (TS) of these species were calculated based on the average standard length. The estimated biomass of fish in the Gulf of Thailand for the pre-monsoon and the post monsoon seasons were 9.2 x 105 tones (i.e. 5.5 x 105 tones of pelagic fish and 3.7 x 105 of demersal fish) and 4.7 x 105 tones (i.e. 2.0 x 105 tones of pelagic fish and 2.7 x 105 tones of demersal fish) respectively. The estimated biomass of fish off the east coast of Peninsula Malaysia for the pre-monsoon and post-monsoon seasons were 4.4 x 105 tones (i.e. 2.3 x 105 tones of pelagic fish and 2.1 x 105 tones of demersal fish) and 3.2 x 105 tones (i.e. 1.9 x 105 tones of pelagic fish and 1.2 x 105 tones of demersal fish) respectively.